【摘要】 根据GenBank上的Ghrelin基因的序列设计了2对引物,用PCR-SSCP方法检测了140只京海黄鸡和30只苏禽黄鸡的该基因的多态性。结果表明,引物1在两个鸡品种中均未检测到多态;引物2检测到多态:其中京海黄鸡有BB、BC和CC3种基因型;苏禽黄鸡中只检测到BB和BC两种基因型。在2个鸡品种中B等位基因为优势基因,分布较高,其中在苏禽黄鸡中BB基因型频率达0.933,而京海黄鸡为0.657。对两个纯合基因型测序分析表明,位于Ghrelin基因546bp处发生了T→A的单碱基突变。更多还原

【Abstract】 Two primers were designed based on the Ghrelin gene sequence in GenBank,and the polymorphism of Ghrelin gene in 140 Jinghai yellow chickens and 30 Suqin yellow chickens was analyzed by PCR-SSCP.The results indicated that there were no polymorphism detected by Primer 1,and there were polymorphisms detected by Primer 2.BB,BC and CC genotypes were detected in Jinghai yellow chicken,but only BB and BC genotypes were detected in Suqin yellow chickens;the allele B was obviously higher than that of C allele in two chicken breeds.The frequency of BB genotype was 0.933 in Suqin yellow chickens and 0.657 in Jinghai yellow chickens.The sequencing results showed that there was one single nucleotide mutation(T→A)at the point of 546 bp of Ghrelin gene in yellow chickens.更多还原