【摘要】 目的探讨多药耐药(MDR)基因产物P-糖蛋白(P-gp)、谷胱甘肽-S-转移酶-π(GST-π)及DNA拓扑异构酶Ⅱ(TopoⅡ)在乳腺癌组织中的表达及其相互关系。方法采用免疫组织化学方法,检测184例未经抗肿瘤治疗的原发性乳腺癌组织中P-gp、GST-π及TopoⅡ表达水平。结果乳腺癌组织中P-gp、GST-π和TopoⅡ阳性表达率分别为49.51%、45.65%和73.91%,TopoⅡ阳性表达率明显高于P-gp及GST-π阳性表达率(P<0.05);2种多药耐药基因产物共表达率为30.98%,2种和3种多药耐药基因产物共表达率为67.39%,明显高于单独表达率12.41%(P<0.01);P-gp与GST-π的表达呈显著正相关(P<0.05),P-gp和TopoⅡ的表达呈明显负相关(P<0.05),GST-π和TopoⅡ的表达呈明显负相关(P<0.05)。结论P-gp、GST-π及TopoⅡ在乳腺癌组织中表达对肿瘤耐药起重要作用,单基因和多基因协同作用,以多基因共表达为主。更多还原

【Abstract】 Objective To explore expression of multidrug resistance (MDR) gene production of P-glycoprotein (P-gp), Glutathione-s-transferase -π (GST-π) and Topo Ⅱ in breast cancer tissue. Methods A total of 184 case with untreated primary breast carcinoma were detected immunohistochemically for P-gp, GST-π and TopoⅡ. Results The positive expression rate of P-gp, GST-π and TopoⅡ separately were 49.51%,45.65% and 73.91%. The positive expression rate of TopoⅡ was much higher than that of P-gp、GST-π (P<0.05). The coexpression rate of only 2 MDR gene production and 3 MDR gene production was 30.98% and 67.7%, respectively. Those coexpression rates were higher than individual expression rate (12.41%). There was significantly positive correlation beteen P-gp and GST-π (P<0.05) and there was significantly negative correlation beteen P-gp related and TopoⅡ(P<0.05). GST-π had obviously negative correlation with TopoⅡ(P<0.05). Conclusion The expression of P-gp,GST-π and TopoⅡ in breast cancer tissue play an important role in tumor drug-resistance. There is synergistic action between single gene and multiple genes.更多还原