【摘要】 参考Gen Bank公布的NADL-2株序列,设计出1对引物,并利用该引物扩增了猪细小病毒NJ-1株VP2主要抗原基因,将其克隆到pGEM-T Easy载体上,测序获得882 bp核苷酸序列,并推导出其氨基酸序列,共编码294aa,与NADL-2株相应的序列进行比较分析,两者核苷酸同源性为99.2%,氨基酸同源性为99%;应用DNAStar软件对氨基酸的抗原表位进行了预测,共有9个抗原表位,分别在氨基酸N端的第34-40,62-70,88-92,137-157,167-181,189-200,206-219,258-272和280-294区段,此序列具有较好的免疫原性.更多还原

【Abstract】 Designing a pair of primers according to NADL-2 strain of PPV from the Genbank,the major antigen regions of VP2 of NJ-1 strain were amplified by PCR.The PCR products were cloned into pGEM-T Easy vector.882 bp nucleotide sequence was acquired,and 294 amino acid sequence was deduced.Comparing it with NADL-2 strain correspondingly,the homologies of nucleotide and amino acid sequences of the two strains were 99.2% and 99% respectively.The prediction of VP2 antigen epitopes using DNAstar softcware showed that nine antigen epitopes were located at the segments of 34～40,62～70,88～92,137～157,167～181,189～200,206～219,258～272 and 280～294 in amino acid N terminal.This sequence has a better immuning originality.更多还原