【摘要】 目的:探讨肺腺癌原发灶及其淋巴结转移灶中凋亡相关基因bcl-2及p53的表达意义,为进行肺腺癌的分子生物学研究提供理论依据。方法:采用免疫组化S-P法,用抗bcl-2单克隆抗体及抗p53单克隆抗体分别对63例发生淋巴结转移的肺腺癌组织及其相应淋巴结进行标记分析,且按性别、年龄、分化程度、TNM分期等进行分组比较。结果:bcl-2与肺腺癌淋巴结转移、患者性别、年龄、分化程度无关(P>0.05),TNM分期Ⅰ~Ⅱ期肺腺癌bcl-2阳性率大于Ⅲ~Ⅳ期肺腺癌,差异具有显著性(P<0.05);p53与肺腺癌淋巴结转移、患者性别、年龄无关(P>0.05),与分化程度有关(P<0.05),TNM分期肺腺癌p53阳性率Ⅲ~Ⅳ期高于Ⅰ~Ⅱ期肺腺癌,差异具有显著性(P<0.05)。结论:bcl-2及p53在肺腺癌发生发展过程中可能起一定作用,可作为评估肺腺癌预后的生物学指标。更多还原

【Abstract】 Objective:To investigate the significance of the expression of bcl-2 and p53 in lung adenocarcinoma and lymph node metastasis.Method:The expression of bcl-2 and p53 in 63 cases of lung adenocarcinoma with lymph node metastasis were analysed by-immunohistochemical SP method using monoclonal antibody to bcl-2 and monoclonal antibody to p53.Results:bcl-2 was not related to lymph node metastasis,patients’sex,age and differentiation in lung adenocarcinoma(P>0.05):the positive rate of bcl-2 was higher in TNM stage Ⅰ～Ⅱ than in stage Ⅲ～Ⅳ(P<0.05);p53 was not related to lymph node metastasis,patients’sex,age in lung adenocarcinoma(P>0.05).p53 was correlated with differentiation(P<0.05).The positve rate of p53 was higher in TNM stage Ⅲ～Ⅳ than in stage Ⅰ～Ⅱ(P<0.05).Conclusions:bcl-2 and p53 may play role in the carcinogenesis of lung adenocarcinoma.They can be valuable in predicting the prognosis of lung adenocarcinoma.更多还原