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1种快速提取转基因冰草基因组DNA的方法
A RAPID METHOD FOR EXTRACTION GENOME DNA FROM TRANSGENIC WHEATGRASS PLANTS
【摘要】 本文介绍了1种改良SDS法提取转基因冰草基因组DNA的方法。该方法具有快捷、高效、经济等特点,所提取的DNA纯度符合分子生物学操作要求,可用于转基因冰草植株的PCR检测及Southern检测等。
【Abstract】 A improved SDS method was used to extract genome DNA from transgenic hybrid wheatgrass(Agropyron cristatum×A.desertorum cv.’Hycrest-Mengnong’).This method had the advantages of rapid,high efficient and economics.The DNA quality can match the need of molecule-biology operation,such as PCR analysis and Southern analysis for transgenic plants.
【关键词】 转基因冰草;
DNA提取;
DNA纯度;
【Key words】 Transgenic hybrid wheatgrass; DNA extraction; quality of DNA;
【Key words】 Transgenic hybrid wheatgrass; DNA extraction; quality of DNA;
【基金】 国家转基因植物研究与产业化专项(J-2002-B-008)
- 【文献出处】 内蒙古农业大学学报(自然科学版) ,Journal of Inner Mongolia Agricultural University(Natural Science Edition) , 编辑部邮箱 ,2007年02期
- 【分类号】S54
- 【被引频次】1
- 【下载频次】111