【摘要】 目的:鉴定骨髓间充质干细胞(MSCs)异体移植后是否分化为“类精子”。方法:①制作睾丸去势的动物模型:取4周龄雄性白色BALB/c小鼠40只,用环磷酰胺腹腔注射法制作睾丸绝育的动物模型,随机分成实验组和对照组各20只;②制备种子细胞(MSCs):取5~6周龄雄性灰色129小鼠10只,以Percoll密度梯度离心法和贴壁法相结合的方法分离、培养、纯化MSCs。待细胞生长至数量足够多时,用生理盐水制备成浓度为1×106/ml的细胞悬液;③异种系小鼠MSCs睾丸异体移植:实验组用盲打法给每只小鼠睾丸注射上述制备的129小鼠的MSCs悬液。对照组睾丸注射生理盐水;④移植后的观察:另取8~10周龄雌性白色BALB/c小鼠40只,分别与实验组和对照组的40只雄性小鼠按雌雄1∶1合笼,观察雌鼠受孕情况。结果:实验组:有8只雌鼠分别于合笼后31~46(平均38.5)d怀孕,受孕率为40%,产下的小鼠皆为白色。对照组:1只雌鼠怀孕,受孕率为5%,产下的小鼠为白色。子代鼠皆为白色,提示129小鼠的MSCs未能分化为有功能的“类精子”,使BALB/c小鼠的子代携带129小鼠的基因而呈现预想中的黑白相间的“花色”。两组受孕率差异有显著性(P<0.05),提示MSCs对睾丸损伤有促进愈合作用。结论:异品系的MSCs睾丸移植后未能分化为有功能的“类精子”,但对睾丸损伤有促进愈合作用。更多还原

【Abstract】 Objective: To determine whether the surviving mesenchymal stem cells (MSCs) in the testis after transplantation can differentiate into quasi-sperm. Methods: ① Making an animal model with sterilized testes. Forty 4-week old white male BASB/C mice were used to establish an animal model with sterilized testes and divided randomly into an experimental and a control group. ② Cell preparation. The MSCs from 10 gray male 129-mice were isolated, cultured and purified by Percoll density gradient centrifugation combined with the adherent method. When the MSCs grew to an adequate number, they were made into a cell suspension with NS at a concentration of 1 million cells/ml. ③ Xenogeneic transplantation of the MSCs into the testis. The MSC suspension was blindly injected into the testes of the mice in the experimental group and NS into the testes of the controls. ④ Post-transplantation observation. Forty white female BASB/C mice were adopted, each put into a box with a male mouse from the experimental group or the control group, and then observed for pregnancy. Results: In the experimental group, 8 cases of pregnancy (40%) were observed at 31-46 d (38.5d on average), the offspring all white. In the control group, only 1 case of pregnancy (5%) was seen at 45d, the offspring all white, too. It was suggested that the MSCs of the 129-mice failed to differentiate into functional quasi-sperm and pass their genes to their offspring, as would expectedly have been presented by a mixture of black and white. The pregnancy rates of the two groups were significantly different (P<0.05), which indicated that MSCs could promote the healing of the testis damage. Conclusion: MSCs cannot differentiate into quasi-sperm after heterogeneity transplantation into the testis, but can promote the healing of the testis damage.更多还原