【摘要】 目的:研究不同浓度的刺五加水提物体外对弱精子症患者精子运动的影响,探讨其可能的作用机制。方法:将35例弱精子症患者经手淫法获得并通过上游优化处理的精子,与不同浓度刺五加水提物共同孵育30、60、120、180 m in后,应用计算机辅助精子分析系统(CASA)观察不同浓度(2.5、5、10、20 g/L)刺五加水提物对人精子各运动参数的作用。结果:不同浓度刺五加水提物能明显提高弱精子症患者精子的运动能力,在浓度为5 g/L和10 g/L时,精子活率(MOT)、前向运动精子百分率、曲线运动速度(VCL)、直线运动速度(VSL)和平均路径速度(VAP)与空白对照组相比,差异均有显著性(P<0.05)。结论:刺五加水提物在体外能明显改善弱精子症患者精子运动能力,其最佳浓度为10 g/L。更多还原

【Abstract】 Objective: To study the effect of different concentrations of the extract of acanthopanacis senticosi on human sperm motility in vitro and to investigate its possible mechanism.Methods: By computer-assisted sperm analysis(CASA) system,we observed the effect of different concentrations of the extract of acanthopanacis senticosi on human sperm motility in vitro.The sperm obtained by masturbation and prepared by swim-up technique from 35 men with asthenospermia was incubated in different concentrations of the extract of acanthopanacis senticosi,and all the specimens were measured at 30,60,120 and 180 min respectively.Results: Different concentrations of the extract of acanthopanacis senticosi obviously improved the sperm motility of asthenospermia patinets.The extract at the concentrations of 5 and 10 g/L increased the rate of motility(MOT),the percentage of progressive mobile sperm,the curvilinear velocity(VCL),the straight line velocity(VSL) and the average path velocity(VAP).Compared with the control group,the difference was significant(P<(0.05)).Conclusion: The extract of acanthopanacis senticosi can improve the sperm motility of asthenospermia patients in vitro and its optimal concentration is 10 g/L.The study may provide a new drug therapy for asthenospermia.更多还原