【摘要】 对2005年采自河北省10个县(市)的130株小麦丝核菌及采自6个县(市)的21株玉米丝核菌进行了生物学测定,结果表明不同的培养基成分对病菌的生长有明显的影响,菌株在各种培养基上的生长速度为:PDA培养基>玉米粉培养基>水琼脂培养基。在pH值为4~9时丝核菌均能良好生长,pH值为6时生长速度最快;通过番红O-KOH染色观察发现130株小麦分离菌株中有双核丝核菌122株,占93.85%,多核丝核菌8株,占6.15%;21株玉米分离菌株中双核丝核菌2株,占9.52%,多核丝核菌19株,占90.48%;选取典型的丝核菌菌株进行了盆栽小麦、玉米相互接种的致病性测定,结果表明,不同类型的菌株间致病力强弱存在明显差异,且丝核菌对原寄主的致病力强。更多还原

【Abstract】 The biological characteristics of 130 and 21 Rhizoctonia isolates collected from wheat and corn in different areas of Hebei province in 2005 were tested.The result showed that the Rhizoctonia growth on different medium was clearly different.The order of growth speed on different medium was PDA media>maize media>water agar media.The Rhizoctonia can grow well on PDA medium with pH 4-9,the optimum pH value was pH6.The O-KOH dyeing results indicated that 122 isolates out of 130 Rhizoctonia isolates from wheat were Rhizoctonia cerealis with percentage of 93.85%,8 isolates were Rhizoctonia solani with percentage of 6.15%;2 isolates and 19 isolates out of 21 Rhizoctonia isolates from corn were Rhizoctonia cerealis with percentage of 9.52% and Rhizoctonia solanis with percentage of 90.48%,respectively.The pathogenicity of some typical isolates was valuated by artificial cross-inoculation.The results showed that the virulence of different isolates were different,the virulence of Rhizoctonia on original host was higher than that on the other hosts.更多还原