【摘要】 目的:研究普乐可复(即FK506)对肝癌肝移植术后体内可能残存肿瘤细胞的影响,从而对肝癌患者肝移植术后抗排异药物的选择起一定的指导作用。方法:在体外细胞培养条件下,通过MTT、RT-PCR、Western blot方法,研究普乐可复对肝癌细胞株HepG2增殖及其肿瘤相关细胞因子TGF-α和C-met的特异性表达的影响。结果:普乐可复组(5μg/L)对肝癌细胞株HepG2增殖及TGF-α及c-Met特异mRNA及蛋白的表达有抑制作用。与对照组相比,差异均有统计学意义(P<0.05)。结论:FK-506对肝癌细胞生长有一定的抑制作用。更多还原

【Abstract】 Objective:To investigate the effect of prograf on remnant hepatocellular carcinoma cells in the liver receptors after liver transplantation for liver cancer,and to instruct the anti-rejection drug selection.Methods:MTT assay,RT-PCR and Western blot were used to examine the expression of c-met and TGF-α in the hepatocellular carcinoma cell line HepG2 cultured in vitro.Results:The expression of c-met and TGF-αin prograf group(at concentration of 5 μg/L) was much lower than that in the control group(P < 0.05).Conclusion:Prograf can inhibit the proliferation of hepatocellular carcinoma cell.更多还原