【摘要】 目的:检测GATA-4基因在大鼠胚胎心脏中的蛋白表达变化。方法:取大鼠胚胎12、15、19天心脏,应用Westernblot、免疫组化的方法进行半定量、定位分析。结果:免疫组化显示,GATA-4基因在房室肌、心内膜、心内膜垫、房室瓣及大动脉根部均有表达。Western blot显示,在胚胎12天心脏中,GATA-4基因已有较高表达;在胚胎15天表达明显上调,胚胎19天时表达又有所下降。结论:GATA-4随大鼠胚胎心脏的生长发育呈动态表达,GATA-4与心脏发育密切相关。更多还原

【Abstract】 Objective:To investigate the protein expression of GATA-4 in the developing rat hearts.Methods:Embryolc hearts of normal rats were obtained on the 12 th,15 th and 19 th day during embryonic period.The expression level of GATA-4 protein was accessed by Western blot and Immunohistochemistry,and the results were analyzed by the statistical method of One-way ANOVA.Results:GATA-4 was expressed in atrial ventricular myocardium,endocardium,endocardial cushion and the root of main artery,The expression of GATA-4 in the heart was increasing obviously on 15 th day in embryonic period,but it was slightly decreasing on 19 th day.Conclusion:With the development of rat hearts,the protein expression of GATA-4 was dynamic.It was suggested that GATA-4 should play a crucial role in rat heart development.更多还原