【摘要】 目的:观察氯胺酮雾化吸入对哮喘模型大鼠气道高反应性的影响。方法:40只Brown Norway大鼠随机分成对照组(C组)、哮喘模型组(A组)、氯胺酮1组(K1组)、氯胺酮2组(K2组)、氯胺酮3组(K3组),应用动物体描箱法测定大鼠的气道反应性,采用RT-PCR反应测定核因子-кB(NF-кB)的基因表达,采用免疫组化的方法观察NF-кB在支气管上皮的表达。结果:在乙酰胆碱(ACH)浓度为50、100、200μg/kg时,K1、K2、K3组呼气阻力(Re)的增长率明显小于A组(P<0.01);在ACH浓度为50、100、200μg/kg时,K1、K2、K3组肺动态顺应性(Cldyn)的下降率明显小于A组(P<0.01)。大鼠肺NF-кB p65 mRNA的表达水平和支气管上皮NF-кB阳性细胞率A组显著高于C组(P<0.05),治疗组K1、K2、K3明显低于A组(P<0.05)。结论:氯胺酮雾化吸入治疗可明显减轻哮喘模型大鼠气道高反应性。更多还原

【Abstract】 Objective:To investigate the effect of inhaled ketamine on airway hyperresponsiveness in allergic asthma rats. Methods:Forty male Brown Norway rats were randomized into five groups:control group C,asthma groups A, ketamine pretreatment group K1,K2 and K3. Airway responsiveness were assessed using a whole body plethysm graphy RT-PCR was used to dectect the mRNA expression of NF-κB. NF-κB levels in bronchioles of all groups were measured by immunohistochemistry. Results:The increase of resistence of exhalation(Re) in group K1,K2 and K3 was significantly lower than that in group A with a dose of 50 μg/kg,100 μg/kg,200 μg/kg of acetylcholine(ACH)(P < 0.01). The decrease of Cldyn in group K1,K2 and K3 was significantly compared with group A with a dose of 50 μg/kg,100 μg/kg,200 μg/kg(P < 0.01) of ACH. NF-кB P65 mRNA expression and NF-кB levels in asthma group was significantly higher than that of control group(P < 0.05), and that of K1,K2,K3 group significantly lower than that of asthma group(P < 0.05). Conclusion:Ketamine aerosol could effectively alleviate the airway hyperresponsiveness in allergic asthma rat.更多还原