【摘要】 目的:比较产前给予沐舒坦(盐酸氨溴索注射液)、地塞米松对大鼠胎肺形态发育的影响。方法:9只孕鼠随机分为生理盐水对照组、沐舒坦治疗组及地塞米松治疗组,每组3只。于孕16、17、18天腹腔注射给药,孕19天每只孕鼠取6只胎鼠肺组织作为早产鼠肺模型,通过光镜观察及电镜技术,分析比较两种药物对胎肺形态发育的影响。结果:光镜下,沐舒坦治疗组、地塞米松治疗组每视野肺泡计数、平均肺泡表面积均高于对照组(P﹤0.01),平均肺泡间隔厚度均低于对照组(P﹤0.01)。地塞米松治疗组每视野肺泡计数、平均肺泡表面积均高于沐舒坦治疗组(P﹤0.01),平均肺泡间隔厚度低于沐舒坦治疗组(P﹤0.01)。透射电镜观察,沐舒坦治疗组、地塞米松治疗组Ⅱ型肺泡上皮内多见板层小体且染色深、致密,胞质内线粒体等细胞器多见,而对照组内难见板层小体、少见细胞器。结论:产前给药沐舒坦、地塞米松均能显著促进胎肺发育,地塞米松效果优于沐舒坦。但考虑到地塞米松的诸多不良反应,沐舒坦可成为临床上促胎肺成熟的更好选择。更多还原

【Abstract】 Objective:To compare the effects of antenatal ambroxol and dexamethason on rat fetal lung morphogenesis. Methods:Nine pregnant rats were divided into three groups randomly:ambroxol group,dexamethasone group and control group with 3 rats in each group. On the 19th day on gestation, cesareans were taken out and the histologic structures of 6 fetal rat lungs of each pregnant rat were observed with light microscope,electronic microscope and image analysis. Results:Under the light microscope, compared with control group,fetal rat lung in the two treatment groups had more alveolar numbers, larger alveolar space, and thinner alveolar septum(P < 0.01). Under the light microscope, compared with ambroxol group,fetal rat lung in the dexamethasone group had more alveolar numbers, larger alveolar space, and thinner alveolar septum(P < 0.01). Under the electronic microscope, lamellar body and cellular organs such as bioblast in endochylema of the two treatment groups were more common compared with the control group. And lamellar body in the two treatment groups was more anachromasis and condensed compared with the control group. Conclusion:Both ambroxol and dexamethasone could promote the development of fetal rat lung, and dexamethasone had prior effects to ambroxol. However, considering of much side effects of dexamethasone, it is suggested that ambroxol could be used as a better drug to promote fetal lung maturity.更多还原