【摘要】 目的:研究选择性血管紧张素Ⅱ受体拮抗剂(缬沙坦)对STZ诱导的糖尿病大鼠血管组织单核细胞趋化蛋白-(1MCP-1)表达的影响。方法:将SD大鼠随机分成三组:正常对照组(NC组)、糖尿病组(DM组)、糖尿病+缬沙坦干预组(DV组)。观察各组大鼠的血糖、体重、Hb1Ac,于8周时用RT-PCR和Westernblot的方法检测链脲佐菌素(STZ)诱导的糖尿病大鼠血管组织中MCP-1的表达的变化。结果:DM和DV组大鼠体重较NC组明显降低(均P<0.01),DM和DV组间糖化血红蛋白(HbA1c)及血糖无显著性差异(P>0.05);DM和DV组大鼠血管组织中MCP-1mRNA的表达明显高于NC组(均P<0.01),而DV组明显低于DM组(P<0.01);DM和DV组大鼠血管组织中MCP-1蛋白的表达明显高于NC组(均P<0.01),而DV组明显低于DM组(P<0.01)。结论:糖尿病SD大鼠血管组织单核细胞趋化蛋白-1(MCP-1)表达明显增加,缬沙坦通过降低血管组织炎症因子MCP-1表达,对糖尿病大血管并发症起防治作用。更多还原

【Abstract】 Objective:To investigate the effects of Valsartan on the expression of monocyte chemoattractant protein-1(MCP-1) in the arteria of STZ-induced diabetic rats. Methods:SD rats were randomly divided into NC group,DM group and DV group. Blood glucose(BG), body weight(BW), and HbA1c were observed. the expression of MCP-1 in the arteria of diabetic rats was detected by RT-PCR and Western blot at the 8 th week. Results:The BW of diabetic rats in DM and DV groups decreased significantly compared with NC group(P < 0.01), but no notable difference was found in HbA1c and BG between DM and DV group(P > 0.05). The expression of MCP-1 mRNA in DM and DV groups was much higher than that in NC group(P < 0.01), but it was much lower in DV group than that in DM group(P < 0.01). The expression of MCP-1 protein in DM and DV groups was much higher than that in NC group(P < 0.01), and it was much lower in DV group than that in DM group(P < 0.01). Conclusion:The expression of MCP-1 is much higher in the arteria of diabetic rats. Valsartan could obviously reduce the expression of MCP-1 in the arteria of diabetic rats and ameliorate the inflammation in the arteria.更多还原