节点文献

磁性纳米四氧化三铁及纳米金协同柔红霉素作用于K562/A02细胞的初步研究

Synergistic effect of magnetic nanopartical Fe₃O₄,Au and daunomycin on K562/A02

推荐 CAJ下载
PDF下载
不支持迅雷等下载工具，请取消加速工具后下载。

【作者】戴永援；陈宝安；王雪梅；张仁云；高峰；丁家华；高冲；孙耘玉；程坚；赵刚；王骏；

【Author】 DAI Yong-yuan1,CHEN Bao-an1,WANG Xue-mei2,ZHANG Ren-yun2,GAO Feng1,DING Jia-hua1,GAO Chong1,SUN Yun-yu1,CHENG Jian1,ZHAO Gang1,WANG Jun1(1.Department of Hematology,Zhongda Hospital,Southeast University,Nanjing 210009,China;2.State Key Lab of Bioelectronics,Southeast University,Nanjing 210096,China)

【机构】东南大学附属中大医院血液科；东南大学生物电子学国家重点实验室；东南大学附属中大医院血液科江苏南京210009；江苏南京210009；江苏南京210096；

【摘要】目的:研究磁性纳米四氧化三铁(Nano-Fe3O4)及纳米金(Nano-Au)是否具有逆转K562/A02细胞耐药的作用。方法:用MTT法测定盐酸柔红霉素(DNR)对K562/A02和K562细胞的毒性,并观察DNR与Nano-Fe3O4或Nano-Au的联合作用。结果:体积分数为25%的Nano-Fe3O4及Nano-Au能有效增强DNR的细胞毒作用,提高细胞的凋亡率,差异具有显著性。结论:Nano-Fe3O4及Nano-Au结合DNR后均能有效逆转K562/A02细胞的耐药性。更多还原

【Abstract】 Objective To determine whether the magnetic Nano-Fe3O4 and Nano-Au can reverse the resistance of K562/A02 cells or not.Methods MTT was used to examine the effects of daunomycin with and without the magnetic Nano-Fe3O4 or Nano-Au on K562 and K562/A02 cells.The intracellular free daunomycin concentration and the apoptosis percentage of the K562/A02 cells were measured by flow cytometry and fluorescent microscope.Results Nano-Fe3O4 or Nano-Au at a 25% volumn ratio raised the intracellular daunomycin accumulation of the K562/A02 cells and induced more apoptosis percentage.Conclusion The Nano-Fe3O4 or Nano-Au with daunomycin can reverse the resistance of K562/A02 cells significantly.更多还原