【摘要】 利用RT-PCR和5′/3′RACE方法,从不结球白菜冷诱导12 h的根中,克隆到1个冷适应相关基因BrLOS2,Gen-Bank登录号DQ334724,其cDNA全长1 666 bp,含有1 335 bp的完整开放阅读框,编码444个氨基酸。BrLOS2基因与芜菁、甘蓝型油菜、拟南芥等物种的烯醇酶基因有80%以上的同源性,有1个烯醇酶N端结构域、1个烯醇酶结构域和保守的DNA结合域。二级结构及三维结构分析表明,BrLOS2具有许多其他LOS家族的共同特性。更多还原

【Abstract】 With RT-PCR and 5′/3′RACE strategies,a cold responsive gene BrLOS2(GenBank accession number DQ334724)was cloned from the roots of Brassica campestris ssp.chinensis,which was cold-induced for 12 hours.The length of its cDNA was 1 666 bp which encompassed an opening-reading frame(ORF)with 1 335 bp encoding 444 amino acids.The homology of the nucleotide sequence of BrLOS2 with Brassica rapa,Brassica napus,Arabidopsis thaliana enolase genes was above 80%.The predicted BrLOS2 gene contained enolase-N domain,enolase domain,conserved putative DNA-binding like LOS2 from Arabidopsis thaliana.The secondary structure and molecular modeling analysis revealed that BrLOS2 strongly resembled other LOS genes.更多还原