【摘要】 检测了转基因Alzheimer病(AD)模型小鼠脑、心、肝、脾、肺、肾、睾丸组织中Cu、Fe、Zn的含量。结果表明:除肾脏外,AD小鼠各组织中Fe的含量都比对照组的高;骨和脑中的Cu比对照组多,其他组织中的Cu比对照组少;脾、肺中的Zn比对照多,其余组织的Zn均少于对照组。AD对过渡元素分布的影响有性别差异:雄性个体中过渡元素含量异常的组织数目比雌性的多,与正常值偏离的程度也比雌性的严重。在脑切片中,AD个体Cu、Zn、Fe的平均含量均高于对照组。对于Cu与Zn,AD组除在顶皮层PtA、梨形-杏仁核皮层区低于对照组外,在皮层其他区域以及丘脑、下丘、海马中均高于对照;AD小鼠脑皮层、海马和下丘脑中的Fe多于对照。试验还发现,脑切片中Cu与Zn的分布地形图有相似性,Fe的分布与Cu、Zn的不同,揭示Cu、Zn有部分共同的运储载体与途径,而Fe的储运途径与之独立。AD诱发的淀粉样蛋白沉聚、血脑屏障通透增大及金属运储载体性能的改变对金属的异常分布起作用。更多还原

【Abstract】 The contents of iron,copper and zinc in kidneys,lungs,hearts,spleens,livers,testicles and brains of APP695,751V717I transgenic mice(Alzheimer′s disease model)were measured with ICP and X-ray induced fluorescence spectrophotometry to make clear the transitional metals′ roles in the pathology of Alzheimer′s disease or vice versa.The data indicated that,compared with the control group,iron contents in lungs,hearts,spleens,livers,testicles and brains of AD mice increased but declined in kidneys;the coppers in bones and brains elevated but decreased in others;zinc contents descended in AD kidneys,hearts,livers,testicles and brains except increase in lungs and spleens.For the male AD sufferers,metals contents changed tissues were more than that of the females.Any average content of copper,zinc and iron in AD brain slices was higher than the control.For detail,the copper and zinc levels in cortex,thalamus,hypothalamus and hippocampus were enhanced,except in parietal and piriform cortex and amygdale nuclear associated area;irons in cortex,hippocampus and hypothalamus of AD brain increased too.The topographic feature of copper was partly similar to that of zinc,but different to iron′s,suggesting copper shared common ways with zinc for transferring and assimilating,freestanding from the iron′s.Amyloid protein and the subsequently formed senile plaques induced by AD,the deterioration of bloodbrain barrier and the damages of metals carriers took roles in the aberrances of metals.更多还原