【摘要】 采用组织分离法从健康的和感染松枯梢病的松树针叶上分离了叶栖微生物1 862株。选取有代表性的菌株通过室内平皿对峙培养,初步筛选出对松枯梢病菌和松赤枯病菌具有较好生防潜力的菌株12株。其中,拮抗细菌菌株6株、酵母菌3株、丝状真菌木霉(Trichoderma sp.)3株,拮抗效果由强到弱依次为细菌、木霉、酵母菌,细菌无菌滤液的拮抗效果明显好于菌体本身。拮抗菌的筛选以相对抑菌效果为准,同时应考虑被抑制率和菌种本身具有的致病性。更多还原

【Abstract】 Through collecting,isolating and cultivating the pine phyllosphere microbes,several isolates were selected to test their antagonistic effect to Sphaeropsis sapinea and Pestalotiopsis sp.By the method of dual culture,the result showed that the isolates with obviously antagonistic ability to Sphaeropsis sapinea and Pestalotiopsis sp.contained six kinds of bacteria,three kinds of yeast and three kinds of Trichodermas sp.,which perhaps could be used for biological control.The antagonistic effect of bacteria is the highest,secondly Trichoderma sp.and yeast lastly.The standard for screening antagonistic fungus is relatively restraining the effect of a fungus.At the same time the restrained proportion and the characteristics leading to disease for the fungus should be considered.更多还原