【摘要】 应用透射电子显微镜研究了杂交鹅掌楸花粉的超微结构形态,并对其花芽发育过程中相关的生理指标做了测定。结果显示:在花药发育初期,败育小孢子母细胞形态异常,细胞质凝聚,细胞器结构模糊,失去细胞分裂功能,不能进行减数分裂。发育正常的小孢子母细胞形态呈圆状或椭圆状,其中细胞质丰富、细胞器结构正常的小孢子母细胞发育成熟后,进入减数分裂形成四分体;而细胞质不丰富或局部细胞器结构退化的小孢子母细胞,虽然可以进入减数分裂,但很难完成,不能形成正常的四分体。同时杂交鹅掌楸花粉的绒毡层细胞形态异常,也造成了花药发育停滞、细胞解体、黏连堆积和畸形生长。这与生理指标中显示的花芽在发育过程中RNA含量、蛋白质含量、氨基酸总量和SOD活性呈下降趋势的结果一致。更多还原

【Abstract】 The ultra-structure of Liriodendron chinense(Hemsl.) Sarg.×L.tulipifera L.pollen was studied by transmission electron microscope.Correlative physiological indexes were determined during the development of bud.The results showed that the form of abortive microspore mother cell was abnormal,the cytoplasm was condensed and the structure of organelle was fuzzy at the initial stage of anther development.Then it lost division function and couldn′t under go meiosis.Generally normal microspore mother cells were round or oval.The cell whose cytoplasm was abundant and organelle structure was clear could complete the normal meiosis.Contrarily,it only could enter meiosis but not finish it.So the normal tetrad didn’t come into being.And the deformed tapetum cell caused stasis of anther development,disintegrating of cells,fusing,piling up and deforming.At the same time,the content of RNA,protein and amino acids reduced constantly in the course of stamen development.更多还原