【摘要】 研究超声波提取核酸的影响因素,并对超声波与食盐法联合提取核酸的方法进行了实验,实验结果表明,最佳条件为:酵母浓度3%,破壁时间为35min,破壁功率为90W,食盐加量为1.3%。用该方法可使核酸的提取率达90%以上。更多还原

【Abstract】 The influencing factors of ultrasonic method for the extraction of nucleic acid were investigated, and the experiments of the use of ultrasonic method combined with salination to extract nucleic acid were carried out to determine the best technical conditions as follows: yeast concentration as 3 %, wall-breaking time as 35 min, ultrasonic power as 90 W, and salt addition level as 1.3 %. Such method could achieve above 90 % extraction rate of nucleic acid.更多还原