【摘要】 双酚A是近年来人们最为关注的一种重要内分泌干扰物,它对生态系统和人类健康会产生很大的危害.采用分子印迹技术,以双酚A为模板分子、2-乙烯吡啶为功能单体、聚三甲基丙烯酸丙三醇酯为交联剂,通过微球法合成了对双酚A具有高度选择性的纳米级印迹聚合物微球.平衡吸附实验表明:与空白聚合物相比,双酚A分子印迹聚合物具有较高的吸附能力和选择识别能力.通过Scatchard分析,该聚合物对双酚A存在两类不同的结合位点,其中特异性结合的平衡结合常数达1.78×105M-1,表观最大吸附量为7.23μmol/g.同时比较了该聚合物对双酚A结构相似物的吸附行为,结果表明MIPs对双酚A具有更好的特异选择性和识别能力,为复杂介质中双酚A的检测提供了一种快速准确有效的方法.更多还原

【Abstract】 Bisphenol A (BPA) has been receiving more and more attention in recent years because it has serious effects on the endocrine systems for ecosystem and human health. In this paper, the molecularly imprinted technology (MIT) was employed to synthesize a molecularly imprinted polymer (MIP) for BPA recognition. A series of molecularly imprinted nano-polymers for bisphenol A has been successfully fabricated by using different functional monomers, cross-linkers, initiators and porogens. The result showed that MIP with high selectivity for bisphenol A was obtained by selecting bisphenol A as the template molecule, 2-vinylpyridine as the functional monomer, azobisisobutyronitrile (AIBN) as the initiator, toluene as the porogen and trimethylolpropane trimethacrylate (TRIM) as the cross-linker. The synthesis conditions of the imprinting polymers were optimized and their selective binding characteristics were evaluated by Scatchard analysis. The multiple-site binding model was used to calculate the maximum number of binding sites and the favorable association constant. The results indicated that bisphenol A imprinted polymers had higher affinity character, compared with non-imprinted polymers. Scatchard analysis demonstrated that two different classes of the binding sites were formed in imprinted polymers which had different affinity to bisphenol A. The favorable association constant for bisphenol A binding to be KB=1.78×105 M-1. Density of the bisphenol A-specific receptor sites in the MIPs material was 7.23 μmol/g. The selective binding experiment for similar structure substrates revealed that the bisphenol A imprinted polymers gave much higher affinity and selectivity for bisphenol A than that for Phenol and Tetrabromobisphenol A. This study suggests that MIT may be a potential technique in the future for the selective separation, enrichment and determination of trace bisphenol A in complex environmental matrix.更多还原