【摘要】 为了建立测定磺酰脲除草剂甲磺隆残留的酶联免疫吸附检测方法(ELISA),以邻甲酸甲酯苯磺酰胺与琥珀酸酐反应合成半抗原1-[(2-甲酸甲酯)苯磺酰]单胺琥珀酸.用活性酯化法将半抗原分别与牛血清蛋白(BSA)和卵清蛋白(OVA)偶联制备免疫抗原和包被抗原.通过免疫抗原免疫新西兰大白兔获得甲磺隆的多克隆抗体,抗体效价达800 000.以此抗体建立的间接竞争ELISA法,检测甲磺隆的线性范围在0.7~40 mg/L,最低检测限为0.16mg/L.与甲磺隆结构相似的磺酰脲类除草剂的交叉反应结果表明,氯嘧磺隆的交叉反应率为38.2%,其他4种除草剂的交叉反应率都小于1%.更多还原

【Abstract】 In order to establish a competitive enzyme linked immunosorbent assay(ELISA) for the sulfonylurea herbicide residual of metsulfuron-methyl in food and environment,a hapten,1-[(2-methylester) phenylsulfonyl] monoamidosuccinic acid,was synthesized using 2-carbomethoxybenzenesulfonamide with succinic acid.The hapten was coupled respectively to carrier proteins BSA and OVA by the activated ester method.Polyclonal antibody against metsulfuron-methyl was raised in two New Zealand white rabbits.The titers of the antiserum reached 800 000. Using the hapten-OVA conjugate as the coating agent the indirect competitive ELISA was conducted with the antiserum.The linear response range for metsulfuron-methyl was between 0.7～40 mg/L and the limit of detection was 0.16 mg/L.The results of cross-reactivity of five sulfonylureas showed that chlorimuron-ethyl was 38.2%,and the other four were less than 1%.This study provided a basis of setting the standard immunoassay for the metsulfuron-methyl residual.更多还原