【摘要】 从鱼塘沉积物中驯化、筛选出能以氯霉素为惟一碳源和能源生长的好氧混合菌CSFO,经过平板分离纯化和进一步筛选得到了对氯霉素有较好降解效果的纯菌株CSFO-3,对其进行了形态特征、生理生化、16SrDNA序列分析和7d生长及降解效果研究。结果表明,经过10代的驯化,筛选出的好氧混合菌CSFO在底物浓度为100mg·L-1时7d的降解率为28.96%,分离纯化得到的CSFO-3菌株7d内降解率达30.01%,其菌液在降解4d时菌密度达最大,该菌株经鉴定为假单胞菌属(Pseudomonas Migula),与铜绿假单胞菌(Pseudomonas aeruginosa)的同源性达99%。更多还原

【Abstract】 An aerobic mixed culture CSFO that degraded chloramphenicol (CAP) was enriched from aquaculture pond sediment samples. CSFO could use CAP as sole carbon and energy source for growth. A bacterial strain CSFO-3 was isolated from CSFO, it could degrade CAP better than CSFO. CSFO-3 was identified as Pseudomonas Migula by its morphology, physiological characters, biochemical characters, and 16S rDNA sequence analysis. The growth of CSFO-3 and the degradation of CAP were also studied. The results indicated that the mixed culture CSFO could degrade CAP after the domestication procedure was repeated 10 times. The degradation rate of CAP by mixed culture CSFO with initial concentration of 100 mg·L-1 in 7d was 28.96%, being 30.01% by strain CSFO-3. The bacterial density reached the peak after 4 d incubation. The strain CSFO-3 was considered to be Pseudomonas aeruginosa since they exhibited 99% similarity in partial 16S rDNA sequence.更多还原