【摘要】 目的探讨3型鼠肝炎病毒(MHV-3)感染不同品系小鼠所致不同临床疾病类型小鼠肝炎模型后体内T细胞亚群的动态变化。方法取Balb/cJ和A/J小鼠各8只,腹腔注射100pfu3型鼠肝炎病毒(MHV-3),采用流式细胞仪检测感染后0、24、48、72h外周血、脾细胞悬液以及肝脏组织中T淋巴细胞亚群包括CD3+CD4+CD8-、CD3+CD4-CD8+及CD3+CD4-CD8-T(DNT)细胞的细胞数及各自在T淋巴细胞中所占比率。结果Balb/cJ小鼠于感染后24h外周血、脾脏以及肝脏T淋巴细胞中DNT细胞比率明显升高直至小鼠3d后全部死亡,CD3+CD4+CD8-和CD3+CD4-CD8+T淋巴细胞比率相应下降;A/J小鼠感染后24～96h外周血和脾脏T细胞各亚群变化不明显。结论两种不同品系小鼠感染MHV-3后的不同转归及DNT细胞比率的不同改变提示DNT细胞可能在病毒性肝炎发生、发展中起着一定的作用。更多还原

【Abstract】 Objective To study the changes of T cell subsets in different MHV-3 induced viral hepatitis. Methods A total of 16 mice (8 Balb/cJ, 8 A/J) were injected intraperitoneally with MHV-3 of 100 PFU. Flow cytometry was used to detect the T cell subset ratio of total T lymphocytes in blood, spleen, and liver at 0, 24, 48, and 72 h post MHV-3 infection. Results The CD3+CD4-CD8- T cell ratio in Balb/cJ mice was raised significantly since 24 h post MHV-3 infection and reach the peak until day 3. The CD3+CD4+CD8- and CD3+CD4-CD8+ T cell ratios degraded accordingly. However, the T cell subsets ratios in A/J mice have no significant changes. Conclusion The diffe-rences of DN T cell ratio and prognosis between Balb/cJ and A/J mice after MHV-3 infection suggest that DN T cell might play an important role in the development of fulminant viral hepatitis.更多还原