【摘要】 目的在外源抗原表位表达载体系统中构建并表达HBV抗原表位,并对其抗原性进行研究。方法人工合成编码HBV“a”抗原决定簇表位中24个氨基酸(S124-147)的寡核苷酸序列,克隆入外源抗原表位表达载体系统FHV-RNA2-I位点,构建重组质粒,在原核pET系统(BL21细胞)中表达。以表达产物为包被抗原对其抗原性进行研究。结果嵌和蛋白与抗-HBs血清可发生特异性结合反应。结论在外源抗原表位表达系统中表达的HBV抗原表位具有良好的抗原性。更多还原

【Abstract】 Objective To construct and express HBV recombinant epitope in a foreign epitope presenting vector, and to study antigenicity of HBV epitope. Methods Oligonucleotide sequences encoding 24 amino acids (aaS124-147) within ’a’ antigenic determinant were synthesized and inserted into a foreign epitope-presenting carrier I3 site (FHV-RNA2-cDNA-I3). HBV epitope was expressed in a prokaryotic cellular system (BL21 cell). The expression product was analyzed and its antigenicity was further studied by ELISA and Western blot method with chimeric protein as coating antigen. Results The chimeric protein could reacted with anti-HBs sera specially. Conclusion HBV epitopes expressed in a foreign epitope-presenting carrier possess good antigenicity.更多还原