【摘要】 以常规棉中棉所23为对照,采用饲喂法,测定了转双价(Bt+CpTI)基因抗虫棉中棉所45不同器官对亚洲玉米螟幼虫的杀虫活性和对幼虫的生长抑制作用,调查了田间罩笼试验和自然为害的结果。结果表明:中棉所45对亚洲玉米螟幼虫都具有较高的抗性。室内饲喂结果还表明,对一代的抗性>二代>三代;对1龄抗性>3龄;对3龄幼虫的抑制生长作用显著。更多还原

【Abstract】 A transgenic Bt+CpTI cotton,CCRI 45,was used to test the insecticidal activity and inhibition of different organs to larvae of O.furnacalis using feeding bioassay.Bioassays in lab and with cages in fields were conducted,and field population and the damage of O.furnacalis were surveyed.The results of Bioassay in lab showed that the mortality of O.furnacalis of three generations feeding on different organs of CCRI 45 was higher than those feeding with CCRI 23,the resistance to the first generation of O.furnacalis was higher than that to the second.The resistance to the third generation was the lowest.The resistance to the first generation instar was higher than that to the third.The inhibition to the growth of third generation instar larvae of O.furnacalis feeding with different organs of CCRI 45 was significant.Adjusted decreasing rate of larvae weight of O.furnacalis from first to third generation were 57.58%,49.58%,31.65%,respectively.The result of cages experiments in fields showed that the numbers of holes in stem,buds and bolls of each CCRI 23 plant damaged by the second generation of O.furnacalis were 9.60,1.00,5.40,respectively,and larval survival rate of O.furnacalis was 16.00%.The numbers of holes in stem and bolls of each CCRI 23 plant damaged by the third generation of O.furnacalis were 2.00,4.00,respectively,and larval survival rate of O.furnacalis was 11.43%.No damage and no insect of O.furnacalis of any stages were found in CCRI 45.The result of field population and the damage of O.furnacalis showed that the number of larvae per plant and the rate of damaged plants of CCRI 23 were 0.4,40.00,respectively.Our experiments clearly demonstrated that,the resistance of the bivalent cotton CCRI 45 to larvae of O.furnacalis was higher than CCRI 23.更多还原