【摘要】 目的:测定趋化因子受体CX3CR1 mRNA在银屑病患者中的表达水平及其与PASI之间的关系。方法:应用RT-PCR法检测了33例斑块状银屑病患者皮损处、非皮损处皮肤和淋巴细胞及其中16例治疗后患者淋巴细胞中CX3CR1 mRNA的表达水平,设30例健康人为对照组;将检测结果与PASI及皮损处与淋巴细胞中该受体的表达水平分别进行相关性分析。结果:CX3CR1mRNA表达水平:在银屑病治疗前与治疗后外周血淋巴细胞中分别为1.64±1.19与2.15±1.30,均明显高于对照组(0.72±0.72,P<0.001);在银屑病皮损处表皮中为1.33±0.40,明显高于对照组(0.89±0.26,P<0.001)及非皮损处皮肤(0.94±0.45,P<0.01);在银屑病皮损处真皮中为1.59±0.76,明显高于对照组(0.89±0.41,P<0.001)及非皮损处皮肤(1.21±0.53,P<0.01),并与PASI(r=0.601,P<0.001)及淋巴细胞中该受体的表达水平之间呈正相关(r=0.351,P<0.05),非皮损处真皮中CX3CR1 mRNA表达水平也高于对照组(P<0.05)。结论:CX3CR1可能主要通过参与活化、趋化淋巴细胞而在银屑病的发病机制及病理状态的维持中起到重要作用。更多还原

【Abstract】 Objective: To investigate the expression of chemokine receptor CX3CR1 mRNA in patients with progressive plaque psoriasis and its correlation with psoriasis area and severity index (PASI). Methods: Reverse transcriptase-polymerase chain reaction (RT-PCR) technique was applied to semi-quantitatively analyze CX3CR1 mRNA expression in epidermis and dermis from lesional and non-lesional skin, and peripheral blood lymphocytes in 33 patients with psoriasis vulgaris before treatment and 30 normal controls. Of the 33 patients the expression of chemokine receptor CX3CR1 mRNA in lymphocyte was measured in 16 patients with about 70% remission after treatment. The correlations between CX3CR1 mRNA values and PASI, and between the CX3CR1 mRNA values in lymphocytes and in epidermis and dermis were evaluated, respectively. Results: CX3CR1 mRNA levels in lymphocytes from pre-treated and post-treated psoriatic patients were 1.64±1.19 and 2.15±1.30, respectively, both markedly higher than that in normal controls (0.72±0.72,P<0.001). CX3CR1 mRNA level in psoriatic epidermis was 1.33±0.40, markedly higher than that in normal controls (0.89±0.26,P<0.001)and psoriatic non-lesional epidermis(0.94±0.45,P<0.01). CX3CR1mRNA level in psoriatic lesional dermis was 1.59±0.76, markedly higher than that in normal controls (0.89±0.41,P<0.001)and non-lesional dermis (1.21±0.53,P<0.01), and the CX3CR1mRNA level in non-lesional dermis was also higher than that in normal control (P<0.05). Positive correlations were noticed between CX3CR1 mRNA levels and PASI (r=0.601,P<0.001), and the expression of CX3CR1 mRNA levels between dermis and lymphocytes (r=0.351,P<0.05). Conclusion: The increased expression of CX3CR1 in psoriasis may be associated with migration and aggregation of lymphocytes in psoriatic lesion, suggesting that this chemokine receptor be involved in the pathogenesis of progressive plaque psoriasis.更多还原