【摘要】 目的研究VEGF-C反义寡核苷酸对胰腺癌原位种植瘤模型中PANC-1细胞凋亡的影响。方法建立人胰腺癌细胞株PANC-1裸鼠原位种植瘤自发性淋巴结转移模型,分离原代培养原位灶和淋巴结转移灶中的胰腺癌细胞,并应用荧光定量PCR、流式细胞术检测VEGF-C反义寡核苷酸转染对其凋亡能力的影响。结果VEGF-C反义寡核苷酸抑制胰腺癌细胞VEGF-C的表达后,可促进淋巴结转移灶中PANC-1细胞的凋亡,而对原位灶中胰腺癌细胞无明显影响(P>0.05)。结论VEGF-C反义寡核苷酸能显著促进淋巴结转移灶中胰腺癌细胞的凋亡。更多还原

【Abstract】 Objective To investigate the effect of antisense oligonucleotide(AS-ODN) of vascular endothelial growth factor C(VEGF-C) on apoptosis of pancreatic cancer PANC-1 cell. Methods Establishment of the spontaneous lymphatic metastasis model in nude mice with orthotopic implantation for the human pancreatic cancer cell line PANC-1,isolation and culture of primary tumor and spontaneous lymphatic metastasis.The effect of VEGF-C ASODN on apoptosis of pancreatic cancer cell derived from primary tumor and spontaneous lymphatic metastasis were detected by real-time quantitative polymerase chain reaction(PCR) and flow CytoMeter. Results Transfection of VEGF-C ASODN decreased the expression of VEGF-C on pancreatic cancer cell,the apoptosis of pancreatic cancer cell derived from spontaneous lymphatic metastasis was increased significantly(P<0.05),but pancreatic cancer cell derived from primary tumor was not effected (P>0.05). Conclusion Pancreatic cancer cell derived from spontaneous lymphatic metastasis promoted apoptosis by transfection of VEGF-C ASODN specially,but no effect on primary tumor.更多还原