【Abstract】 With the completion of the Populus genome sequence in 2006,the study on functional genomics in Populus has become a major task.Establishment of Populus mutant population is an essential approach for forestry functional genomics study.The activation tagging is a promising and powerful method to construct mutant library of Populus and to further discover new gene and elucidate its function now.A novel T-DNA activation tagging pCAS05 was constructed for forest functional genomics in this study.We could select conveniently and easily transgenic plants by spraying Basta because bar gene is constructed in this vector as a selection marker.Moreover,we could also construct a large scale mutant library in Populus by this activation tagging because of effectively selection of bar gene.更多还原