【摘要】 目的:研究丹参酮ⅡA磺酸钠(sodium tanshinoneⅡ-A sulfonate,DS-201)对单侧输尿管梗阻(UUO)病人肾间质纤维化来源的成纤维细胞(hRIFs)体外增殖及细胞周期素D1(cyclin D1)蛋白表达的影响,探讨该药治疗肾间质纤维化的作用机制。方法:体外培养鉴定hRIFs;用四甲基偶氮唑(MTT)法检测对照组与不同DS-201浓度组hRIFs的增殖活性;用免疫细胞化学SABC法和图像分析技术检测对照组与不同DS-201浓度组hRIFs cyclin D1基因的表达。结果:随药物浓度的升高和作用时间的延长,抑制率逐渐升高,抑制作用逐渐增强,呈剂量依赖性和时间依赖性;用药组阳性细胞的光密度值在第3、5、7、9天显著低于对照组(P<0.05,P<0.01,﹚第1天各组之间无统计学差异(P>0.05)。结论:①DS-201对UUO病人hRIFs体外增殖有显著抑制作用,可能是其治疗肾间质纤维化的机制之一;②DS-201抑制UUO病人hRIFs体外增殖可能是通过抑制细胞中cy-clin D1基因表达,延长细胞周期实现的。更多还原

【Abstract】 Objective: To discuss the effect of sodium tanshinone II-A sulfonate(DS-201)on human renal interstitial fibrocytes(hRIFS) of UUO and the expression of cyclin D1 and approach the possible mechanism.Methods:HRIFs were assessed through culture in vitro;the propagate activity of HRIFs of control group and different concentration group of DS-201 was detected by MIT method.The expression of cyclin D1 were analyzed with immune cytochemistry SABC and the image-analytical technique.Results:With the increase of drug concentration and action time,the inhibition ratio and strength increased,and it was dose-dependent and time-dependent.The average optical density of positive cell of drug-using group was much less than control group on the third,fifth,seventh,and ninth day(P<0.05,P<0.01).There were no significant differences between groups on 1st day(P>0.05).Conclusions: ①DS-201 can significantly suppress in vitro generation of hRIFs,this may be one of its mechanisms to treat renal interstitial fibrosis.②DS-201 suppress in vitro generation of hRIFs is possibly through inhibiting the expression of cyclin D1 and extending the cell cycle.更多还原