【摘要】 目的观察血管紧张素Ⅱ受体阻断药氯沙坦对急性心肌梗死后左心室心肌肌球蛋白重链(myosin heavy chain,MHC)基因表达的影响。方法Sprague-Dawley大鼠24只随机分为3组,正常对照组,急性心肌梗死组,氯沙坦急性心肌梗死组,每组8只。急性心肌梗死造模后第2日开始灌胃给药,口服氯沙坦日剂量3mg/kg,6周后测量左心室重量、进行心肌肌球蛋白重链基因分析。结果急性心肌梗死组大鼠心脏重量指数明显增加,氯沙坦治疗后心脏重量指数明显下降(P<0.01);Northern plot发现,急性心肌梗死组大鼠心肌细胞的α-MHC mRNA表达量明显下降,而β-MHC mRNA的表达量则显著上升;而氯沙坦治疗组大鼠心肌的α-MHC mRNA表达较急性心肌梗死组增加,α-MHC mRNA表达明显降低(P<0.01)。结论氯沙坦能减轻梗死心肌肥厚,改善梗死心肌心室重构,机制与其调节心肌MHC的基因表达有关。更多还原

【Abstract】 Objectives To investigate the protective effects of losartan in the gene expression of myosin heavy chain(MHC)after acute myocardial infarction(AMI). Methods Twenty-four SD rats were randomly divided into 3 groups (8 rats each group) as following: The control group, the AMI group, and the AMI group with oral prescription of 3 mg/kg losartan per day. At the 6th weeks, the left ventricular weight was measursd, and gene analysis of myosin heavy chain was carried out. Results Compared with contrast group, the left ventricular mass index was significantly increased (P<0.01) in AMI group, but was significantly decreased (P<0.01) after treated with losartan. Northern blot showed that the expression of β-MHC mRNA obviously decreased, while the expression of β-MHC mRNA increased in AMI group. But the expression of β-MHC mRNA significantly increased and the expression of β-MHC mRNA significantly decreased (P <0.01) after treated with losartan. Conclusions The data suggested that losartan might significantly prevent left ventricular remodeling following AMI in rats. This function was associated with regulating of gene expression of cardiac MHC.更多还原