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榛子种质资源遗传多样性的RAPD分析
Analysis of genetic diversity in Corylus by RAPD markers
【摘要】 利用RAPD分子标记技术,对来自欧洲、美国和我国北方的共48份榛子品种或品系(包括4个种及种间杂种)的遗传多样性进行了分析.从150条随机引物中筛选出24条引物,共扩增出142条清晰可重复的带,其中多态性带130条,比例为91.5%.聚类分析将48份榛子种质分为5组:欧榛、平榛各自列为1组;杂交榛分成2个组;另外一组包括滇榛和藏榛2份种质.研究结果对榛子的种间亲缘关系进行了验证,且为同种不同品种的亲缘关系提供了更为详细的信息.
【Abstract】 Random amplified polymorphic DNA(RAPD) analysis was carried out to measure the genetic diversity of 48 accessions of Corylus(include 4 species),which were collected from Europe,America and Northern China.Twenty-six polymorphic primers were screened out of 150 random primers and 142 bands were distinguishable and reproducible.Among them,130 bands(91.5%) were polymorphic.The 48 accessions were divided into 5 groups according to the cluster analysis.Corylus avelana and C.heterophylla belonged to the individual group.The hybrid Corylus were divided into 2 different groups.The other group contained C.yunnanensis and C.ferox var.thibetica.Our results were consistent with the traditional taxonomy and provided more valuable information for Corylus accessions belonging to the same species.
- 【文献出处】 辽宁师范大学学报(自然科学版) ,Journal of Liaoning Normal University(Natural Science Edition) , 编辑部邮箱 ,2007年02期
- 【分类号】S664.4
- 【被引频次】29
- 【下载频次】260