【摘要】 采用TAIL-PCR技术,对随机选取的6个HDF-68阳性T-DNA转化子进行扩增,只利用1 d的时间,即获取了T-DNA标签侧翼序列(FSTs),对其序列进行分析后,分析了影响TAIL-PCR扩增的主要因素.该项研究,为从T-DNA的突变子中分离紫杉醇产生菌产紫杉醇的相关基因提供了一条可行途径,也为阐明紫杉醇产生菌的代谢途径以及最终构建高产基因工程菌株奠定了基础.更多还原

【Abstract】 TAIL-PCR technique was performed to get the flanking sequence of T-DNA tags(FSTs) from six randomly selected HDF-68 positive T-DNA transformants only in one day time.After analysis of its sequences,the main factors of influencing TAIL-PCR amplification were discussed.This study provided a feasible way to seperate taxol biosynthesis related genes for taxol-producing fungi from T-DNA mutants,and also laid solid foundation for clarifying taxol pathway in taxol-producing fungi and for finally constructing high-yield gene engineering strain.更多还原