【摘要】 根据人凝血酶原复合物(Prothrombin Complex Concentrate,以下简称PCC)的性质,对合成的大孔阴离子交换树脂进行静态吸附研究,并与已经商品化的DEAE Sephadex A50、Streamline DEAE凝胶型离子交换树脂进行比较,筛选出吸附性能较优的CG-6树脂。通过静态吸附实验,测定了CG-6对PCC在不同温度下的吸附等温线、考察了吸附过程中的动力学和热力学行为并初步探讨了吸附机理。结果表明,吸附动力学可用拟二级速率方程来描述,计算值与实测值吻合较好。CG-6对PCC的吸附等温线可分为两段,在较低的平衡浓度下,吸附平衡符合Langmuir方程,温度的升高有利于平衡吸附量的增大,表明该吸附反应为一吸热过程。求出反应的焓变△H=115.1kJ/mol,熵变△S分别为2.37J/mol·K(5℃)、20.97J/mol·K(15℃)、23.11J/mol·K(20℃)、37.32J/mol·K(30℃),吉布斯自由能变化△G分别为-0.54kJ/mol(5℃)、-5.93kJ/mol(15℃)、-6.66kJ/mol(20℃)、-11.20kJ/mol(30℃)。此外,除了离子交换,CG-6对PCC也有物理吸附现象。更多还原

【Abstract】 According to properties of PCC, a series of anion exchangers were made, from which CG-6 resin was selected because of its better adsorption properties in static studies compared with the commercial DEAE Sephadex A50、Sreamline DEAE gels. The adsorption isotherms of PCC on CG-6 was measured at different temperature. Through static experiment, the kinetic and thermodynamic parameters of the adsorption were investigated, and the adsorption mechanisms were also studied. The results showed that the adsorption kinetics can be described by pseudo second order model and adsorption capacity calculated by the model was consistent with the actual measurement. The adsorption isotherms of PCC could be divided to two parts. The experimental datas with lower PCC equilibrium concentration followed both Langmiur isotherm models and the adsorption capacity increased with increasing temperature, which indicated that the adsorption process was endothermic. For the adsorption process, the enthalpy was 115.1kJ/mol, the entropy was 2.37kJ/mol·K (5℃), 20.97kJ/mol·K (15℃), 23.11kJ/mol·K (20℃), 37.32kJ/mol·K (30℃), and the free energy was -0.54kJ/mol (5℃), -5.93kJ/mol (15℃), -6.61kJ/mol (20 ℃ ) and -11.20kJ/mol (30 ℃ ), respectively. Research on the adsorption mechanism showed that physical adsorption existed during the adsorption process except the ion exchange process.更多还原