【摘要】 研究了小分子染料罗丹明S与热变性脱氧核糖核酸(DNA)在弱酸性条件下共振光散射光谱的特征。考察了各种影响因素,在优化条件下确立了共振光散射强度与鱼精DNA和小牛胸腺DNA浓度之间的线性关系,相应的线性范围分别为0.024~3.00,0.018~3.50 mg.L-1;相关系数为0.998 6,0.999 0;检出限为24.0,18.3μg.L-1;基于共振光散射的增强,建立了一种测定DNA的方法。更多还原

【Abstract】 Based on the study of resonant scattering spectrometric characteristics of the reaction of thermo-denatured DNA with small molecular dyestuff rhodamine S(RDMS) in B-R buffer medium of pH 5.0,a new method for determination of DNA was proposed.Effects of 20 co-existing substances on the determination of DNA were studied,and the tolerance limits found were higher than their actual amounts present in the organisms.Under the optimum condition of the reaction,linear relationships between the magnitude of enhancement of intensity of resonant scattering and the concentrations of fish sperm DNA and calf thymus DNA were obtained in the ranges of 0.024-3.00 mg·L-1(r=0.998 6) and 0.018-3.50 mg·L-1(r=0.999 0) respectively.Detection limits were found to be 24.0 μg·L-1 for fish-sperm DNA and 18.3 μg·L-1 for calf thymus DNA.更多还原