【摘要】 [目的]探讨分离培养和聚合酶链反应(PCR)在硫酸盐还原菌类群分析鉴定中的应用。[方法]采用分离培养和PCR对贵州阿哈湖沉积物中硫酸盐还原菌类群进行分析,并对分离纯化的一株硫酸盐还原菌进行鉴定。[结果]贵州阿哈湖沉积物硫酸盐还原菌类群为脱硫肠菌属、脱硫叶菌属和脱硫球菌-脱硫线菌-脱硫八叠菌属;纯化菌株经PCR扩增初步鉴定为脱硫叶菌属,菌株形态与鉴定结果相符。[结论]采用分离培养结合PCR,可以对硫酸盐还原菌类群进行分析和鉴定。更多还原

【Abstract】 [Objective] To study the utilization of isolation culture and PCR in the identification of sulfate-reducing bacteria subgroups. [Methods] Isolation culture and PCR were performed to analyze sulfate-reducing bacteria subgroups in the sediment of Lake Aha in Guizhou Province and a purified strain identified from the sample. [Results] Desulfotomaculum, Desulfococcus- Desulfonema-Desulfosarcina and Desulfobulbus were detected in the sample. The purified strain was demonstrated preliminarily be Desulfobulbus and the morphologic characteristic of the strain is congruous with Desulfobulbus. [Conclusion] The analysis and identification of sulfate-reducing bacteria subgroups can be achieved by isolation culture and PCR.更多还原