【摘要】 目的建立一种测定人体血浆中奥沙普秦含量的方法,并应用于奥沙普秦肠溶片的药动学研究。方法采用Agilent HPLC系统;色谱柱:Diamoniltm C18柱(150 mm×4．0 mm,5μm)。流动相为乙酸缓冲液(20 mmol·L-1,pH 4．0):甲醇=23:77,流速1．0 mL·min-1,λ=280 nm。结果奥沙普秦线性关系为Y=16．32×ρ-9．03(n=7,r=0．999 7);线性范围0．04～80 mg·L-1;最低检测限为0．012 mg·L-1;日内、日间RSD分别<5．05％、9．75％。结论本方法简便、准确、灵敏,可以作为奥沙普秦肠溶片血药浓度监测的有效方法。更多还原

【Abstract】 AIM To establish a method to determine the concentration of oxaprozin in human plasma which can be applied to study the pharmacokinetics of oxaprozin enteric tablets. METHODS Agilent HPLC instrument was used with the column:Diamonsiltm( 150 mm×4.0 mm,5μm) .The mobile phase was composed of NaAc(20 mmol·L-1,pH 4.0):CH3OH(23:77, V/V) .Flow rate was 1.0 mL·min-1.Detection wavelength was 280 ran. RESULTS The linear coefficient relation of oxaprozin was Y - 16.32×ρ-9.03(n = 7,r = 0. 999 7) . The minimum detection limit of oxaprozin was 0.012 mg·L-1 .The coefficient variations of intra-and inter-day were less than 5.05% and 9.75% . CONCLUSION The methed is practical and accurate to monitor the serum drug levels of oxaprozin enteric tablets.更多还原