【摘要】 目的探讨鼠骨骼肌肌源干细胞(MDC)自体移植在压力性尿失禁治疗中的可行性。方法采用差速贴壁法分离、纯化培养6只雌性大鼠的MDC,体外脂质体介导法转染pEGFP-N1质粒入MDC,转染成功后将筛选的MDC注射于自体膀胱颈和尿道周围,5、15 d处死大鼠切取膀胱及后尿道,组织学检查观察注射组织形态学改善,免疫组化检测GFP蛋白的表达,观察MDC存活情况。结果5、15 d注射部位组织未见明显炎症改变及炎性细胞浸润,局部细胞层数增多,免疫组化显示各时间点大量细胞细胞质内可见棕黄色GFP蛋白表达,且在5、15 d两个时间点GFP阳性细胞数量和染色强度没有明显改变。结论MDC自体膀胱尿道周围注射可持续存活,自体MDC有可能成为治疗压力性尿失禁的一种理想材料。更多还原

【Abstract】 Objective To explore the feasibility of muscle-derived cells(MDC) autologous transplantation in the treatment of stress urinary incontinence.Methods Muscle-derived cells were isolated and purified from 6 female Sprague-Dawley rats by replate technique,and then the cells were transduced with plasmid pEGFP-N1 by lipofectamine.MDCs transduced successfully were injected into the bladder wall and proximal urethra of 6 autologous animals.Tissue was harvested and sectioned after 5 and 15 days,and then histological examination and immunohistochemistry were performed.Results Histological examination revealed no apparent sign of inflammation and inflammatory cells infiltration,but there were more layers of cells locally in the bladder and urethral wall 5 and 15 days after autologous cell injection in Sprague-Dawley rats.A large number of cells expressing GFP protein was observed by immunohistochemical stain and the persistence of primary MDCs at 5 days was similar to that at 15 days.Conclusion The MDCs autologous transplantation can survive permanently,indicating that autologous MDCs transplantation may be a potential treatment of stress urinary incontinence.更多还原