【摘要】 目的:探讨β-纤维蛋白原基因-455G/A多态性与中青年脑梗死的关系。方法:应用聚合酶链反应-限制性片段长度多态性分析法(PCR-RFLP)对78例脑梗死患者和62名健康对照人群进行β-纤维蛋白原基因-455G/A多态性分析。结果:在脑梗死组GG基因型、GA基因型和AA基因型者分别为0.67、0.29和0.04。对照组依次分别为0.72、0.25和0.03。脑梗死组和对照组两组间比较差异无统计学意义(P>0.05)。A等位基因频率在患者组和对照组分别为0.19和0.15,经统计学分析两组间比较,差异也无统计学意义(P>0.05)。结论:本研究表明山西地区汉族人群中,β-纤维蛋白原基因-455G/A多态性与脑梗死无相关性。更多还原

【Abstract】 Aim:To explore the relationship of β-fibrinogen gene -455G/A polymorphism and susceptibility of cerebral infarction in young and middle-aged patients. Methods:Genomic DNA in 78 subjects with cerebral infarction and 62 healthy controls were purified with DNA purification kits. Polymerase chain reaction of restricted fragment length polymorphism (PCR-RFLP) method was applied to determine the bi-allelic polymorphism in β-fibrinogen gene -455G/A . Results:The genotypes of A/A, G/A and G/G were 4%, 29% and 67% in cerebral infarction patients, and were 3%, 25% and 72% in controls(P>0.05). The A allele frequency of allele was 19% and 15% in cerebral infarction patients and controls respectivrely(P>0.05). Conclusion:The β-fibrinogen gene-455G/A polymorphism had no relation to the cerebral infarction patients.更多还原