【摘要】 目的探讨N5,10-亚甲基四氢叶酸还原酶(MTHFR)基因C677T位点、内皮型一氧化氮合酶(eNOS)基因G894T位点与2型糖尿病合并脑梗塞的关系。方法采用Sequenom系统检测内蒙古地区汉族健康对照组65人、2型糖尿病患者34例、2型糖尿病合并脑梗塞患者42例的MTHFR、eNOS基因型。结果(1)eNOS基因G894T位点2型糖尿病合并脑梗组TT基因型频率、T等位基因频率与对照组比较差异有显著性(P<0.01,P<0.01);2型糖尿病合并脑梗组T等位基因频率与糖尿病组比较差异有显著性(P<0.05),(2)MTHFR基因C677T位点的TT基因型与eNOS基因G894T位点的TT基因型在2型糖尿病人群患脑梗塞方面具有协同作用(P<0.05)。结论MTHFR基因C677T位点和eNOS基因G894T位点变异增加糖尿病患者发生脑梗的危险性,可能是糖尿病患者发生脑梗塞的遗传易感基因。更多还原

【Abstract】 Objectives To investigate the association between C667T point mutation of N5,10-methylene tetrahydrofolate reductase(MTHFR) gene,G894T point mutation of endodermis nitricoxide synthase(eNOS) gene and the patients with type 2 diabetes mellitus(T2DM)complicating cerebral infarction(CI).Methods Sequenom system was used to test Han nation people’s MTHFR、eNOS genotype of 65 control groups、34 patients withT2DM and 42 patients withT2DM complicating CI in inner Mongolia.Results ⑴eNOS gene G894T site’s frequencies of TT homogenesis and T allele in patients with T2DM complicating CI are significently higher than those in control,there is significent difference(P<0.01,P<0.01);the frequencies of T allele in patients with T2DM complicating CI there is significent difference compare with T2DM(P<0.05).⑵TTgenotype of eNOS gene G894T site and MTHFR gene C677Tsite have synergistic effect in the patients with T2DM complicating CI(P<0.05).Conclusion MTHFR gene C677T and eNOS gene G894T site mutation increase chanciness of T2DM proceeding CI,they may be Hered predisposing genes.更多还原