【摘要】 把混合发酵技术应用于分离自南海红树植物的2株内生海洋真菌(菌株编号为1924#和3893#)的培养,其发酵液乙酸乙酯粗提物表现出明显的杀棉铃虫(Heliothisarmigera Hühner)和中华鳋(Sinergasilus spp.)的活性。从该粗提物分离到2个纯培养未能得到的次级代谢产物A和B,经波谱分析分别鉴定它们为2-甲基水杨酸(A)和环(苯丙-苯丙)二肽(B)。其中优势代谢产物A是粗提物具有杀虫活性的主要原因。更多还原

【Abstract】 Mixed fermentation technique was applied to culture two marine-derived mangrove endophytic fungi (strains Nos. 1924# and 3893#) from the South China Sea in this paper. The crude extracts from the fermentation liquids extracted by ethyl acetate showed significant bioactivities of killing pests Heliothis armigera Hühner and Sinergasilus spp. in the primary bioassay. Two secondary metabolites A and B were isolated from this crude extracts, and elucidated by spectral methods as 6-methylsali-cylic acid(A) and cyclo(phe-phe)dipeptide(B) respectively. Compounds A and B were not obtained from these two strains by pure fermentation. Compound A was responsible for the significant biocide activities of the crude extracts.更多还原