【摘要】 目的观察冠心病(CHD)患者血浆脂联素(APN)与血管内皮功能指标内皮素-1、一氧化氮(NO)、假血友病因子(vWF)水平变化并分析其相关性。方法分别采用酶联免疫吸附法、放射免疫分析法、硝酸还原酶法测定28例年龄和性别相当的健康体检者和60例CHD患者血浆APN和vWF、内皮素-1、NO水平,其中冠心病患者包括35例稳定型心绞痛(SAP)和25例急性冠状动脉综合征(ACS)的患者,比较各组间的差异有无统计学意义。结果冠心病患者血浆APN与NO均显著低于健康对照组,分别为(7.79±3.22)mg/L和(11.35±2.90)mg/L;(41.57±14.73)μmol/L和(67.51±18.69)μmol/L(P<0.01),而血浆内皮素-1、vWF则显著高于健康对照组(81.65±23.07)ng/L和(52.17±13.65)ng/L;(119.3±54.59)%和(96.45±32.10)%(P<0.05~0.01);其中ACS患者血浆APN、NO均显著低于SAP(6.66±3.10)mg/L vs(8.60±3.09)mg/L;(35.73±15.82)μmol/L vs(45.74±12.53)μmol/L(P<0.05~0.01),而血浆内皮素-1、vWF均显著高于SAP(89.49±25.53)ng/L vs(76.05±19.65)ng/L;(136.63±53.94)%vs(106.95±52.35)%(P<0.05)。血浆APN与内皮素-1呈显著负相关(r=-0.522,P<0.01),与NO则呈显著正相关(r=0.415,P<0.01),与vWF无明显相关性(r=-0.129,P>0.05)。结论冠心病患者血浆APN、NO水平显著降低,而内皮素-1与vWF则与之相反,以ACS为著;血浆APN可能参与内皮功能的调节。更多还原

【Abstract】 Objective To investigate the plasma level of adiponectin,endothelin-1(ET-1),nitric oxide(NO) and von Willebrand factor(vWF) in the patients with coronary heart disease and their relationship.Methods Plasma level of adiponectin and vWF,ET-1,NO in 28 healthy outpatients(healthy control group) and 60 patients with coronary heart disease(CHD) were detected by enzyme-linked immuneosorbent assay(ELISA),radioimmunoassay(RIA) and enzymatic assay with nitrate reductase respectively.Sixty patients with CHD were divided into two groups:stable angina pectoris(SAP) group(n=35) and acute coronary syndrome(ACS) group(n=25).Results Adiponectin and NO in the patients with CHD were significantly lower than those in healthy control group,respectively(7.79±3.22) mg/L vs(11.35±2.90) mg/L;(41.57±14.73) μmol/L vs(67.51±18.69) μmol/L all P<0.01,while ET-1 and vWF were significantly higher,(81.65±23.07) ng/L vs(52.17±13.65) ng/L;(119.3±54.59)% vs(96.45±32.10)%(P<0.05-0.01),and the sameresults were observed between ACS group and SAP group,adiponectin(6.66±3.10) mg/L vs(8.60±3.09) mg/L(P<0.05);NO(35.73±15.82) μmol/L vs(45.74±12.53) μmol/L(P<0.01);ET-1(89.49±25.53) ng/L vs(76.05±19.65) ng/L(P<0.05);vWF(136.63±53.94)% vs(106.95±52.35)%(P<0.05).The level of adiponectin was negatively correlated with that of ET-1(r=-0.522,P<0.01) and positively correlated with that of NO in plasma(r=0.415,P<0.01),while adiponectin was irrelated with vWF(r=-0.129,P>0.05).Conclusion The plasma level of adiponectin and NO decreased significantly in the patients with CHD,while those of ET-1 and vWF increased significantly.Plasma adiponectin may play a role in the protection of endothelial function.更多还原