【摘要】 目的探讨类风湿性关节炎(RA)患者T细胞表面协同刺激分子表达与其发病的相关性及临床意义。方法采用流式细胞术分析RA患者外周血T淋巴细胞表面共刺激分子CD28、CD152和CD154的表达。结果RA患者外周血CD4+T细胞较正常对照组增加,分别为(41.86±7.67)%vs(32.20±6.51)%,CD8+T细胞减少,分别为(23.82±6.88)%vs(28.16±8.43)%,差异均有统计学意义(均P<0.05);协同刺激分子CD28在RA患者较对照组CD4+和CD8+T细胞亚群上分子的表达均减少分别为(9.81±1.36)%vs(42.13±0.59)%和(7.27±3.24)%vs(18.56±2.17)%,而CD152分子的表达均增加,分别为(0.46±1.21)%vs(0.13±0.42)%和(18.78±0.14)%vs(0.42±0.47)%,与对照组比较,差异均有统计学意义(均P<0.05);CD154分子在RA患者CD4+T细胞和CD8+T细胞上表达较正常对照组也均增加,分别为(3.64±2.61%)vs(2.37±1.05)%和(4.23±1.24)%vs(2.13±0.67)%,差异有统计学意义(P<0.05)。结论RA患者机体的免疫功能紊乱与T淋巴细胞表面协同刺激信号分子表达异常具有相关性。更多还原

【Abstract】 Objective To explore the relationship between expression of costimulative molecules on T cells in the patients with rheumatoid arthritis(RA) and their clinical significance. Methods Expressions of costimulative CD28,CD152 and CD154 on T lymphocytes were analyzed by flow cytometry. Results Imbalanced T subsets,increasing CD4+ T cells( 41.86± 7.67)% vs ( 32.20± 6.51)% and decreasing CD8+ T cells( 23.82± 6.88)% vs ( 28.16± 8.43)% were found in the peripheral blood of RA group compared to those of control group(all P< 0.05). Compared to those of control group,the expressions of CD28 on both CD4+T cell and CD8+ T cell in RA group decreased ( 9.91± 1.36)% vs ( 42.13± 0.596)%;( 7.27± 3.24)% vs ( 18.56± 2.17)%, but the expression of CD152 increased ( 0.46± 1.21)% vs ( 0.13± 0.42)%;( 18.78± 0.14)% vs ( 0.42± 0.47)%. The expression of CD28 decreased but the expression of CD152 increased,with significant difference (all P< 0.05).The expressions of CD154 on both CD4+ and CD8+ T cells in RA group increased compared to those of control group ( 3.64± 2.61%) vs ( 2.37± 1.05)%;( 4.23± 1.24)% vs ( 2.13± 0.67)%(P< 0.05). Conclusion Abnormal expressions of costimulative signal molecules on T lymphocytes lead to the imbalanced immune function in the patients with rheumatoid arthritis.更多还原