【摘要】 目的:探讨神经元核心抗原(Neu N)在嗅球和嗅上皮发育过程中的表达特性和意义。方法:在小鼠胚胎发育9.5、11.5、14.5、17.5d,出生当天和3个月成年个体的头部切片标本中,以免疫荧光染色方法检测Ne-uN的表达。结果:刚出生小鼠嗅球内层状结构尚不明显,Neu N表达于嗅球周边区域。在3个月大成年小鼠,嗅球的层状结构已清晰可见,Neu N表达阳性的成熟神经元主要聚集于接近嗅球中心的颗粒细胞层。位于嗅上皮的双极嗅感觉神经元在小鼠胚胎和成年个体中均未见Neu N表达。结论:Neu N通常被认为表达于几乎所有部位的成熟神经元。但在嗅球和嗅上皮中,Neu N只表达于成熟嗅球中的颗粒细胞层。小鼠出生到发育为成熟个体的过程中,Neu N表达阳性的成熟神经细胞由周边逐渐向嗅球中心迁移,可能与气味模式的形成有关。更多还原

【Abstract】 Objective:To study the expression characteristics of NeuN(neuron-specific nuclear protein/neuromal nuclei)in the development of mice olfactory bulb and epithelium.Method:The expression of NeuN in the heads of E9.5,E11.5,E14.5,E17.5,newborn and adult mice were examined by immunofluorescence method.Result:NeuN was expressed in mature neural cells at the margin area of olfactory bulb of new natal mice while the layers of olfactory bulb could not be distinguished.Mature neural cells expressing NeuN aggregated in the granule cells layer,when layers of olfactory bulb of adult mice were clearly formed.The expression of NeuN could not be observed in bipolar olfactory receptor neurons(ORN)in olfactory epithelium.Conclusion:It is usually believed that NeuN is expressed in most mature neurons.However,during the development of olfactory bulb and epithelium,NeuN is expressed only in mature neural cells migrating from margin area to the inner granule cells layer of olfactory bulb.It is proposed that the migration of granule cells in olfactory bulb might be related to the formation of smell pattern.更多还原