【摘要】 目的:研究人喉鳞状细胞癌及其癌旁组织中CTGF和OPN表达水平并探讨两者的相互关系及其临床病理意义。方法:41例喉鳞状细胞癌和20例癌旁组织手术切除标本常规制作石蜡包埋切片,CTGF和OPN染色方法为SP免疫组织化学法。结果:41例鳞状细胞癌CTGF和OPN表达阳性率均为25例(61.0%),其评分值分别为2.41±1.60和3.10±1.63,20例癌旁组织CTGF阳性18例(90%)和OPN阳性3例(15.0%),其评分值分别为4.24±1.42和1.12±0.84,癌组织CGTF阳性率及评分明显低于癌旁组织(χ2=5.44,P<0.05;t=4.36,P<0.01),但OPN则相反(χ2=11.44,P<0.01,t=5.08,P<0.01);颈淋巴结未转移和临床分型T1病例CTGF表达阳性率及其评分明显高于颈淋巴结转移和临床分型T3病例(P<0.01);颈淋巴结转移、临床分型T1及组织学分级Ⅰ级病例OPN表达阳性率及其评分明显低于颈淋巴结转移、临床分型T3及组织学分级Ⅲ级病例(P<0.01);CTGF表达评分与OPN表达评分呈密切负相关(r=-0.56,P<0.01)。结论:CTGF和OPN可能是反映喉鳞状细胞癌进展、生物学行为、转移发生及预后的重要生物学标记物。更多还原

【Abstract】 Objective:To study the expressions of CTGF and OPN and detect their clinical value and correlation in the largngeal squamous cell carcinoma tissues and paracancerous tissues. Method:SP immunohistochemical method was used for the assayes of CTGF and OPN on the routinely paraffin-embedded sections of surgical operated specimens of 41 cases with largngeal squamous cell carcinoma and 20 ones with paracancerous tissues.Result:the positive rate of CTGF and the score were significantly lower in cancer tissues than those in paracancerous tissues (61.0%vs 90.0%, P<0.05; 2.41±1.60 vs 4.24±1.42, P<0.01), but those of OPN wereopposite (61.0% vs 15.0%, P<0.01; 3.10±1.63 vs 1.12±0.84, P<0.01). The positive rates and scores of CTGF were significantly higher in the cases without-metastasis of lymphnode and clinical stage T1 than those in the ones with-metastasis of lymphnode and clinical stage T3 (P<0.01) . The positive rates and scores of OPN were significantly lower in the cases without-metastasis of lymphnode , clinical stage T1 and histological grade Ⅰ those that in the ones with-metastasis of lymphnode, Clinical stage T3 and histological grade Ⅲ (P<0.01). The closely negative correlation was found between the score of CTGF and that of OPN.Conclusion:The expression of CTGF and / or OPN migh be important biological markers in reflecting the progression, biological behaviors, metastatic potential and prognosis of the laryngeal squamous cell carcinoma.更多还原