【摘要】 目的:观察红霉素对上皮细胞凋亡的影响,进一步探讨上皮细胞凋亡在鼻息肉组织形成中的意义。方法:将30例鼻息肉及6例下鼻甲的上皮细胞均分成2组,其中1组加红霉素(红霉素组),另一组不加红霉素(对照组),进行原代上皮细胞的培养,1、3、5d后应用TUNUL法检测细胞凋亡。结果:红霉素组培养1、3、5d的上皮细胞凋亡指数分别为(33.23±6.50)%、(38.21±7.22)%和(52.63±7.86)%,对照组为(31.02±5.60)%、(32.13±7.15)%和(39.64±7.48)%,培养5d的上皮细胞凋亡指数差异有统计学意义(P<0.05)。结论:红霉素有显著的促鼻息肉上皮细胞凋亡的作用。更多还原

【Abstract】 Objective:To study the effect of erythromycin on apoptosis in epithelial cell and investigate the significance of epithelial cell apoptosis in nasal polyps forming.Method:Epithelial cell collected from thirty nasal polyps and six inferior turbinates were cultured in Dulbecco Eagle and Ham F12(1:1)and divided into two groups,one culured with Erythromycin(Erythromycin group),another cultured without Erythromycin control group.Apoptosis was detcted by terminal deoxynucleotidyl transferasemediated dUTP nick endlabelinn.Result:The AI(apoptosis index)of epithelial cell in nasal polyps after cultured for 1,3,5 days with erythromycin were respectively(33.23±6.50)%、(38.21±7.22)% and(52.63±7.86)%.The AI of epithelial cell in inferior turbinates were respectively(31.02±5.60)%、(32.13±7.15)% and(39.64± 7.48)%.There were significant difference between two groups at 5 day after culture(P<0.05).Conclusion:Erythromycin promoted apoptosis of epithelial cell in nasal polyps.更多还原