【摘要】 目的:探讨急性出血坏死性胰腺炎(ANP)时细胞骨架变化和β-胡萝卜素(β-C)二者之间的关系。方法:Wistar大鼠随机分为3组:A组,ANP组;B组,β-C处理组;C组,假手术组,采用Aho方法制作ANP大鼠模型,3组动物分别于30min、1h、6h、处死。以罗丹明—法罗丁(Rhodamine-phalloidin)进行肌动蛋白微丝(F-actin)染色,在共聚焦激光显微镜下观察,荧光值分析。结果:ANP早期就存在细胞骨架的急剧变化和F-actin含量降低。β-胡萝卜素缓解了F-actin细胞骨架的损伤。结论:ANP早期就有细胞骨架的剧烈变化,F-actin分布紊乱、含量降低。β-胡萝卜素缓解了细胞骨架损伤。更多还原

【Abstract】 Objective:To explore the relationship between the change of cytoskeletons and β-carotene in acute hemorrhagic necrosis of pancreas.Methods:Wistar rats were randomly divided into three groups:AHNP group;β-carotene group;control group.After AHNP rat models were made by using the Aho method,the rats of three groups were killed at 30min,1h and 6h, respectively.Immunostained F-actin with rhodamine-phalloidin was analyzed by using confocal laser scanning system.Results:AHNP resulted in early disruption of the cytoskeleton with dramatic changes and F-actin level was decreased.β-carotene moderated the damage of actin-cytoskeleton.Conclusion:The cytoskeleton alteration is a common feature of AHNP,and administration of β-carotene can protect cytoskeleton in acute hemorrhagic necrosis of pancreas.更多还原