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低温下两个品系的钝顶螺旋藻质膜蛋白的变化
Effect of Low Temperature Stress on the PM Proteins of Spirulina (Arthrospira) platensis
【摘要】 采用考马斯亮蓝G-250法和SDS-PAGE电泳对经低温处理和低温锻炼的内蒙古鄂尔多斯沙区碱湖的钝顶螺旋藻(S1)和引进非洲Chad湖的钝顶螺旋藻(S2)的质膜蛋白含量和电泳图谱分析表明:两种藻质膜蛋白对低温反应的规律相似,但抗寒性较强的内蒙古鄂尔多斯沙区碱湖的钝顶螺旋藻(S1)质膜蛋白含量比抗寒性较弱的引进非洲Chad湖的钝顶螺旋藻(S2)的高,较低的低温下质膜蛋白降低幅度S1<S2;直接处理时,抗寒性较强的内蒙古鄂尔多斯沙区碱湖的钝顶螺旋藻(S1)对5℃反应更强。低温锻炼可适当增加螺旋藻的质膜蛋白含量。较低的低温下有分子量较小的新质膜蛋白的合成和分子量较大的旧质膜蛋白的解聚或解体。
【Abstract】 After treated and exercised under low temperature,the content and SDS-PAGE electrophoresis picture of PM proteins of S1 and S2 are analized by coomassie brilliant blue G250 method and SDS-PAGE electrophoresis respectively.The result is that the reaction of PM proteins of S1 and S2 to low temperature is alike,but the content of PM proteins of S1 is higher;the dropping range of PM proteins under lower temperature is S1<S2;S1 has stronger reaction to 5℃ when it is exposed directly to 5℃.The content of PM proteins would increase properly after exercise.There have small proteins appearing and large proteins disappearing at lower temperature.
【Key words】 Spirulina(Arthrospira) Platensis Low temperature stress PM proteins;
- 【文献出处】 科学技术与工程 ,Science Technology and Engineering , 编辑部邮箱 ,2007年07期
- 【分类号】Q946.1
- 【被引频次】4
- 【下载频次】117