【摘要】 目的探讨重组人生长激素(rhGH)对肝脏缺血再灌注损伤中的NF-κB P65蛋白表达的影响.方法Pringle’s法建立100只肝脏缺血再灌注损伤模型,实验组于建立模型前7 d每天给予重组人生长激素(rhGH)针剂皮下注射(0.2 IU/100 g体重),对照组等量的生理盐水注射,应用蛋白质印迹实验(Westernblotting)和免疫组化法检测NF-κB P65蛋白表达水平.结果rhGH组肝细胞NF-κB P65蛋白表达水平及活性均明显低于对照组(P<0.05).结论rhGH能下调大鼠肝脏缺血再灌注损伤中NF-κB P65蛋白的表达,从而减轻肝脏缺血再灌注损伤.更多还原

【Abstract】 Objective To investigate the effect of recombinant human growth hormone(rhGH)on the protein expression of NF-κB P65 in ischemic reperfusion injury of rat liver.Methods One hundred male rats models were constructed by Pringle’s method and were randomly divided into two groups: the rhGH group and the control group.In rhGH group, the rats were injected(0.2IU/100g weight)seven days before the ischemic reperfusion injury,while in control group,rats was replaced by normal sodium.The levels of protein expression of NF-κB P65 were detected by Western blotting and immunohistochemistry.Results Compared with control group,the expression of NF-κB P65 and NF-κB activity(P<0.05)in the liver cells were significantly lower than those in control group.Conclusion rhGH might confer the protection to ischemic reperfusion injury of rat liver through reducing the expression of NF-κB P65.更多还原