【摘要】 初步探讨了空间环境对黑色素瘤B16细胞致瘤基因和蛋白质表达的影响。选择经第20颗返回式卫星搭载的B16细胞,进行体外和体内实验后,筛选出性状变异明显的空间诱变B16细胞株,检测空间诱变B16细胞基因和蛋白质表达的变化。将筛选出的空间诱变B16细胞接种于C57BL/6小鼠,2周后,处死荷瘤小鼠,取出移植瘤,利用免疫组化方法,检测接种空间诱变B16细胞的荷瘤小鼠移植瘤细胞Melan-A和VEGF蛋白的表达情况;利用RT-PCR方法,检测蛋白质水平上表达变化明显的空间诱变B16细胞melan-a,gp100,bag-3和l-pgds基因的表达情况。与对照细胞相比,1株空间诱变B16细胞荷瘤小鼠移植瘤细胞Melan-A和VEGF蛋白表达明显增加,其他细胞2种蛋白质的表达无明显变化。RT-PCR结果显示:此株细胞的melan-a,gp100和l-pgds基因表达增加,bag-3基因表达降低。空间环境的复合因素可诱导B16细胞基因和蛋白质表达产生变化。更多还原

【Abstract】 Preliminary studies were carried out on the effects of space environments on the expression of proteins and genes of melanoma B16 cells. Four B16 cells which were on board of China’s 20th recoverable satellite were selected whose biological characteristics had changed obviously in the experiments in vitro, in order to detect changes of cells’ expression of proteins and genes. B16 cells selected were inoculated into C57BL/6 mice under the chest skin. The tumor mice were killed when space cells had been inoculated for 2 weeks, then, tumors of tumor mice were ablated. To detect the expression of Melan-A(Melanoma Antigen Recognized by T cells 1, MART-1) and Vascular Endothelial Growth Factor(VEGF) in the carcinomas of tumor mice, the method of Immunohistochemistry (IH) was used. According to the above results, the space B16 cells were chosen, whose biological characteristics changed obviously and the expression variations of melan-a, gp100, bcl-2-associated anthanogene-3(bag-3) and Lipocalin-type Prostaglandin D2 synthase (l-pgds) were determined with the method of Reverse Transcription Polymerase Chain Reaction (RT-PCR). It was found that space environments might change the expression of proteins and genes of melanoma B16 cells.更多还原