【摘要】 桔小实蝇Bactrocera dorsalis线粒体基因组全序列对研究实蝇分子系统进化具有重要意义。本研究通过DNA测序和克隆技术,对桔小实蝇mtDNA全序列进行了测定和分析。结果表明:桔小实蝇线粒体基因组全长15915bp(GenBank序列号:DQ845759)。基因组碱基组成为39.3%A,16.2%C,10.2%G,34.3%T,由13个蛋白编码基因、22个tRNA基因、2个rRNA基因以及一个非编码的控制区域(A+T-rich区)组成。7个蛋白编码基因和13个tRNA基因从J链编码,其余6个蛋白编码基因和9个tRNA基因从N链编码。位于J链上的蛋白编码基因具有近似的A、T含量,而位于N链上的蛋白编码基因的A的含量明显高于T的含量。以mtDNACOⅠ基因为例,比较了桔小实蝇与其他14种实蝇的亲缘关系,结果显示其与同亚属(果实蝇亚属Bactrocera)内的其他近缘种相互间的同源性很高。更多还原

【Abstract】 The complete mitochondrial genome of the Oriental fruit fly, Bactrocera dorsalis is important for general molecular and evolutionary studies in Tephritidae. Via sequencing and cloning the target DNA sequences, a complete mitochondrial genome sequence of B. dorsalis from the Chinese population was determined and analyzed. The results indicated that the complete mitochondrial genome of B. dorsalis is a circular molecule of 15 915 nucleotides (GenBank accession no.: DQ845759). Its overall composition is 39.3%A, 16.2%C, 10.2%G, and 34.3%T, and the gene content includes 22 tRNAs, 2 rRNAs and a long non-coding region (A+T-rich region). Seven protein genes and 13 tRNA genes are transcribed from J strand, and the other 6 protein genes and 9 tRNA genes are transcribed genes from N strand. Protein coding genes on J strand show a fairly similar A% and T%, whereas those encoded on N strand have a higher proportion of A than T. The similarity analysis of mtDNA COⅠ genes between B. dorsalis and the other 14 fruit flies showed that there was a high similarity among B. dorsalis and the other species belonging to the same subgenus Bactrocera.更多还原